Faculty Profiles

Brian Stockman

Professor
Chemistry, College of Arts and Sciences

Science Building 34
516.877.4139
bstockman@adelphi.edu

Selected Publications and Presentations

Articles

Articles

Patrone, M., Galasyn, G. S., Kerin, F., Nyitray, M. M., Parkin, D. W., Stockman, B. J. & Degano, M. (2023) A riboside hydrolase that salvages both nucleobases and nicotinamide in the auxotrophic parasite Trichomonas vaginalis, Journal of Biological Chemistry 299, 105077.

Stockman, B. J., Ventura, C. A., Deykina, V. S., Khayan Lontscharitsch, N., Saljanin, E., Gil, A., Canestrari, M. & Mahmood, M. (2023) Direct measurement of nucleoside ribohydrolase enzyme activities in Trichomonas vaginalis cells using 19F and 13C-edited 1H NMR spectroscopy, Analytical Chemistry 95, 5300-5306.

Muellers, S. N., Nyitray, M. M., Reynarowych, N., Saljanin, E., Benzie, A. L., Schoenfeld, A. R., Stockman, B. J. & Allen, K. N. (2022) Structure-guided insight into specificity and mechanism of a parasitic nucleoside hydrolase, Biochemistry 61, 1853-1861.

Auletta, S., Caravan, W., Persaud, J. K., Thuilot, S. F., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) Discovery of ligand-efficient scaffolds for the design of novel Trichomonas vaginalis uridine nucleoside ribohydrolase inhibitors using fragment screening, ACS Omega 4, 16226-16232.

Stockman, B. J., Kaur, A., Persaud, J. K., Mahmood, M., Thuilot, S. F., Emilcar, M. B., Canestrari, M., Gonzalez, J. A., Auletta, S., Sapojnikov, V., Caravan, W. & Muellers, S. N. (2019) NMR-based activity assays for determining compound inhibition, IC50 values, artifactual activity, and whole cell activity of nucleoside ribohydrolases, Journal of Visualized Experiments 148, e59928.

Muellers, S. N., Gonzalez, J. A., Kaur, A., Sapojnikov, V., Benzie, A. L., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) Ligand-efficient inhibitors of Trichomonas vaginalis adenosine/guanosine preferring nucleoside ribohydrolaseACS Infectious Diseases 5, 345-352.

Alam, R., Barbarovich, A. T., Caravan, W., Ismail, M., Barskaya, A., Parkin, D. W. & Stockman, B. J. (2018), Druggability of the guanosine/adenosine/cytidine nucleoside hydrolase from Trichomonas vaginalis. Chemical Biology & Drug Design 92, 1736-1742.

Beck, S., Muellers, S. N., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2015), Adenosine/guanosine preferring nucleoside ribohydrolase is a distinct, druggable antitrichomonal target. Bioorganic & Medicinal Chemistry Letters, 25, 5036-5039.

Stockman, B. J. (2015), Student-led engagement of journal article authors in the classroom using web-based videoconferencing. Journal of Chemical Education, 92, 120-123.

Stockman, B. J., Asheld, J. S., Burburan, P. J., Galesic, A., Nawlo, Z. & Sikorski, K. F. (2014), Design and characterization of a Zn2+-binding four-helix bundle protein in the biophysical chemistry laboratory. Journal of Chemical Education, 91, 451-454.

Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario Jr., I., Parkin, D. W. & Stockman, B. J. (2014), Identification of proton-pump inhibitor drugs that inhibit Trichomonas vaginalis uridine nucleoside ribohydrolase. Bioorganic & Medicinal Chemistry Letters, 24, 1080-1084.

Pacholec, M., Bleasdale, J. E., Chrunyk, B., Cunningham, D., Flynn, D., Garofalo, R. S., Griffith, D., Griffor, M., Loulakis, P., Pabst, B., Qiu, X., Stockman, B., Thanabal, V., Varghese, A., Ward, J., Withka, J. & Ahn, K. (2010), SRT1720, SRT2183, SRT1460, and resveratrol are not direct activators of SIRT1. Journal of Biological Chemistry, 285, 8340-8351.

Sahasrabudhe, P. V. & Stockman, B. J. (2009), Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor. Biomolecular NMR Assignments, 3, 183-186.

Stockman, B. J., Kothe, M., Kohls, D., Weibley, L., Connolly, B. J., Sheils, A. L., Cao, Q., Cheng, A. C., Yang, L., Kamath, A. V., Ding, Y.-H. & Charlton, M. E. (2009), Identification of allosteric PIF-pocket ligands for PDK1 using NMR-based fragment screening and 1H-15N TROSY experiments. Chemical Biology & Drug Design, 73, 179-188.

Stockman, B. J. (2008), 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening. Journal of the American Chemical Society, 130, 5870-5871.

Stockman, B. J., Lodovice, I. J., Fisher, D. A., McColl, A. S. & Xie, Z. (2007), An NMR-based functional assay for NAD synthetase. Journal of Biomolecular Screening, 12, 457-463.

Dalvit, C., Fagerness, P. E., Hadden, D. T. A., Sarver, R. W. & Stockman, B. J. (2003), Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations and range of applicability. Journal of the American Chemical Society, 125, 7696-7703.

Dalvit, C., Hadden, D. T. A., Sarver, R. W., Ho, A. M. & Stockman, B. J. (2003), Multi-selective one dimensional proton NMR experiments for rapid screening and binding affinity measurements. Combinatorial Chemistry & High Throughput Screening, 6, 445-453.

Dalvit, C., Flocco, M., Knapp, S., Mostardini, M., Perego, R., Stockman, B. J., Veronesi, M. & Varasi, M. (2002), High-throughput NMR-based screening with competition binding experiments. Journal of the American Chemical Society, 124, 7702-7709.

Dalvit, C., Flocco, M., Veronesi, M. & Stockman, B. J. (2002), Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures. Combinatorial Chemistry & High Throughput Screening, 5, 605-611.

Dalvit, C., Flocco, M., Stockman, B. J. & Veronesi, M. (2002), Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin. Combinatorial Chemistry & High Throughput Screening, 5, 645-650.

Chapters

Chapters

Stockman, B. J. (2016) NMR-based Activity Assays to Characterize Enzymes in the Biochemistry Laboratory and in Undergraduate Research.  In Soulsby, D., Anna, L. J., Wallner, A. S. (Eds.). NMR Spectroscopy in the Undergraduate Curriculum: Upper-Level Courses and Across the Curriculum Volume 3. (pp. 33-52). Washington, DC: American Chemical Society.

Stockman, B. J. (1996). Preparation of 2H, 13C and 15N Isotopically-Enriched Proteins for NMR Spectroscopic Investigations. In Sarkar, S. K. (Eds.). NMR Spectroscopy and its Application to Biomedical Research. (pp. 159-185). Amsterdam, Netherlands: Elsevier.

Stockman, B. J. & Markley, J. L. (1990). Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution. In Bush, C. A. (Eds.). Advances in Biophysical Chemistry Volume 1. (pp. 1-46). Greenwich, CT: JAI Press.

Stockman, B. J. & Markley, J. L. (1989). Methods of Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution. In Jardetzky, O. (Eds.). Protein Structure and Engineering. (pp. 152-192). New York, NY: Plenum.

Conference Presentations

Conference Presentations

Stockman, B. J., Ventura, C. A., Deykina, V. S., Khayan Lontscharitsch, N., Saljanin, E. , Gil, A., Canestrari, M. & Mahmood, M. (2023) Translation of Nucleoside Ribohydrolase Enzyme Assays for Purified Enzymes to Direct Measurement of Enzyme Activity in Trichomonas vaginalis Cells Using 19F and 13C-edited 1H NMR Spectroscopy, American Society for Biochemistry & Molecular Biology Annual Meeting, Seattle, WA.

Bonilla, J. H., Ventura, C. A. & Stockman, B. J. (2022) Computational Design of Trichomonas vaginalis Purine Nucleoside Hydrolase Inhibitors, American Chemical Society 264th National Meeting, Chicago, IL.

Chimarios, M., Ventura, C. A. & Stockman, B. J. (2022) Parasitology Assays to Assess Trichomonas vaginalis Nucleoside Ribohydrolase Inhibitors, American Society for Biochemistry & Molecular Biology Annual Meeting, Philadelphia, PA.

Saljanin, E., Ajmal, E., Vanegas, D., VanAlstine-Parris, M. A. & Stockman, B. J. (2022) Structure-activity Relationships for Several Series of Fragment-based Inhibitors that Target Trichomonas vaginalis Nucleoside Ribohydrolase Enzymes, American Society for Biochemistry & Molecular Biology Annual Meeting, Philadelphia, PA.

Ventura, C. A., Chimarios, M., Taouil, A., Wang, H., Ojima, I., VanAlstine-Parris, M. A. & Stockman, “B. J. (2022) Concerted Use of Molecular Modeling and Parasitology to Guide Design of Novel Trichomonas vaginalis Nucleoside Ribohydrolase Inhibitors,” American Chemical Society 263rd National Meeting, San Diego, CA.

Mahmood, M., Canestrari, M., Thuilot, S. & Stockman, B. J. (2019) “Translation of 1H and 19F NMR-based Activity Assays to In Vitro Characterization of Nucleoside Hydrolase Activity in Cell Extracts and Whole Cells, American Society for Biochemistry & Molecular Biology Annual Meeting, Orlando, FL.

Canestrari, M., Mahmood, M., Thuilot, S. & Stockman, B. J. (2019) “Translation of 1H and 19F NMR-based Activity Assays to In Vitro Characterization of Nucleoside Hydrolase Activity in Cell Extracts and Whole Cells, American Chemical Society 257th National Meeting, Orlando, FL.

Kaur, A., Gonzalez, J. A., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) “Molecular Modeling and NMR-based Counter Screens of Fragment Inhibitors of Trichomonas vaginalis Adenosine/Guanosine Nucleoside Ribohydrolase,” American Chemical Society 257th National Meeting, Orlando, FL.

Persaud, J. K., Thuilot, S. F., Auletta, S., Caravan, W., Leonardo, A., Li, T., Dulloo, Z., Kabir, N. H., Brown, D. G., Parkin, D. W., VanAlstine-Parris, M. A. & Stockman, B. J. (2019) “Structure-activity Relationships of Fragment-based Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase,” American Chemical Society 257th National Meeting, Orlando, FL.

Thuilot, S. F., Persaud, J. K., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) “NMR-based Counter Screens of Fragment Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Confirm Reversible, Target-specific Inhibition,” American Chemical Society 257th National Meeting, Orlando, FL.

Auletta, S., Caravan, W., Persaud, J., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2018) Identification of Ligand-efficient Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using NMR-based Fragment Screening, American Chemical Society 255th National Meeting, New Orleans, LA.

Caravan, W., Alam, R., Barbarovich, A. T., Ismail, M., Barskaya, A., Parkin, D. W. & Stockman, B. J. (2018) Ribavirin Selectively Inhibits the Guanosine/Adenosine/Cytidine Nucleoside Hydrolase from Trichomonas vaginalis, American Chemical Society 255th National Meeting, New Orleans, LA.

Gonzalez, J. A., Kaur, A., Sapojnikov, V., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2018) Emerging SAR for Several Series of Trichomonas vaginalis Adenosine/Guanosine Preferring Nucleoside Ribohydrolase Inhibitors Derived from Fragment Analog Screening, American Chemical Society 255th National Meeting, New Orleans, LA.

Muellers, S. N., Benzie, A. L., Brown, D. G., Cowen, S., Parkin, D. W. & Stockman, B. J. (2017) Identification of Ligand-efficient Inhibitors of Trichomonas vaginalis Adenosine/guanosine Preferring Nucleoside Ribohydrolase Using NMR-based Fragment Screening, American Chemical Society 253rd National Meeting, San Francisco, CA.

Stockman, B. J., Beck, S., Benzie, A. L., Muellers, S. N., Rosario I., Shea, T. A., Parkin, D. W. & VanAlstine-Parris, M. A. (2016) Two Distinct and Druggable Nucleoside Ribohydrolases as Targets for Trichomonas vaginalis Therapeutic Agents, 17th Tetrahedron Symposium, Sitges, Spain.

Muellers, S. N., Beck, S., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2016) Adenosine/guanosine Preferring Nucleoside Ribohydrolase: From Flavonoids to Fragments, American Chemical Society Middle Atlantic Regional Meeting, Riverdale, NY.

Beck, S., Muellers, S. N., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2016) Adenosine/Guanosine Nucleoside Ribohydrolase is a Distinct and Druggable Antitrichomonal Target, American Chemical Society 251st National Meeting, San Diego, CA.

Shea, T. A., VanAlstine-Parris, M. A. & Stockman, B. J. (2015). Structure-activity Relationships of Prazole Fragment Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using NMR-based Activity and Binding Assays. In 249th American Chemical Society National Meeting. Denver, CO.

Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario I. Jr., Parkin, D. W. & Stockman, B. J. (2014). Identification of Proton-pump Inhibiting Drugs as Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using an 19F NMR-based Activity Assay. In American Chemical Society 247th National Meeting. Dallas, TX.

Stockman, B. J. (2011). 19F NMR-based Activity Assays for Identifying Modulators of Function. In 35th Steenbock Symposium. Madison, WI.

Stockman, B. J., Hadden, D. T. A., Sahasrabudhe, P. V., Herberg, J. T., Hagadorn, J. C., Martin, J. P. Jr. & Rohrer, D. C. (2005). Identification of Ligand Fragments and their Binding Orientation Using WaterLOGSY and 1D Selective Inter-ligand QUIET-NOEs. In XXI International Conference on Magnetic Resonance in Biological Systems. Hyderabad, India.

Sahasrabudhe, P. V., Robarge, J. D., Hadden, D. T. A. & Stockman, B. J. (2002). To Affinity and Beyond: A Functional Genomics NMR Screening Compound Library. In XX International Conference on Magnetic Resonance in Biological Systems. Toronto, ON.

Angwin, D. T., Dalvit, C. & Stockman, B. J. (2001). Empirical Studies of WaterLOGSY and its Range of Applicability for NMR Screening. In 42nd Experimental NMR Conference. Orlando, FL.

Stockman, B. J., Farley, K. A., Angwin, D. T., Schering, C. A., Steinbrecher E. A., Shanmugasundaram, V., Lajiness, M. S., Maggiora, G. M., Bundy, G. L., Marshall, V. P., Yem, A. W., Vosters, A. F., Deibel, M. R. et al. (2000). Screening of Compound Libraries for Protein Binding Facilitated by Flow-Injection NMR Spectroscopy. In XIX International Conference on Magnetic Resonance in Biological Systems. Florence, Italy.

Stockman, B. J., Yuan, P., Waldon, D. J., Scahill, T. A., Sarver, R. W., DeZwaan, J., Belonga, K. L., Jacobsen, E. J., Treiberg, J. A., Schostarez, H. J., Mitchell, M. A., Marshall, V. P., Petzold, G. L. & Poorman, R. A. (1997). Structure, Dynamics and Calorimetry of Stromelysin/Ligand Complexes. In Keystone Symposium: Frontiers of NMR in Molecular Biology V. Taos, NM.

Stockman, B. J., Richardson, T. E. & Swenson, R. P. (1995). Structural Changes Caused by Site-Directed Mutagenesis of Tyrosine-98 in D. Vulgaris Flavodoxin Delineated by 1H and 15N NMR Spectroscopy: Implications for Redox Potential Modulation. In Keystone Symposium: Frontiers of NMR in Molecular Biology IV. Keystone, CO.

Stockman, B. J., Pollock, J. R., Scahill, T. A., Fairbanks, M. B. & Heinrikson, R. L. (1994). NMR Spectroscopy Indicates that the Mac-1 β2 Integrin α-Subunit I domain Adopts a Predominantly Folded Structure in Solution. In XVIth International Conference on Magnetic Resonance in Biological Systems. Veldhoven, Netherlands.

Stockman, B. J., Scahill, T. A., Euvrard, A., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1993). Toward the Solution Structure of Interleukin-1 Receptor Antagonist Protein Determined by Heteronuclear Three-Dimensional NMR Spectroscopy. In Keystone Symposium: Frontiers of NMR in Molecular Biology III. Taos, NM.

Stockman, B. J., Scahill, T. A., Roy, M., Farley, K. A., Ulrich, E. L., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1992). Secondary Structure and Topology of Interleukin-1 Receptor Antagonist Protein Determined by Heteronuclear Three-Dimensional NMR Spectroscopy. In ,” XVth International Conference on Magnetic Resonance in Biological Systems. Jerusalem, Israel.

Invited Presentations

Invited Presentations

Stockman, B. J. (2023, March) Deep Learning Approaches for Exploring the Origin of Oxidoreductases, ENIGMA Seminar Series, Rutgers University, New Brunswick, NJ.

Stockman, B. J., Brown, D. G., Hyatt, I. F. D. & VanAlstine-Parris, M. A. (2021, December) “Fragment-based Drug Discovery Targeting Trichomonas vaginalis Nucleoside Ribohydrolases,” Pacifichem 2021 Congress, Virtual.

Stockman, B. J. (2019, April) NMR-based Activity Assays to Identify and Validate Fragment Leads Against Two Trichomonas vaginalis Enzymes, North Jersey ACS NMR Topical Group, Rutgers University, Piscataway, NJ.

Stockman, B. J., VanAlstine-Parris, M. A., Parkin, D. W., Sonbuchner, T. M., Kumar, R. V. & Hyatt, I. F. D. (2019, April) Multidisciplinary Drug Discovery Research Model at an Undergraduate Institution, American Chemical Society 257th National Meeting, Orlando, FL.

Stockman, B. J. (2019, April) Student-led Safety Inspections of Chemistry Teaching and Research Laboratories, American Chemical Society 257th National Meeting, Orlando, FL.

Stockman, B. J. “(2019, March) Academic Honesty Awareness Week at Adelphi University,” International Center for Academic Integrity 27th Annual Conference, New Orleans, LA.

Stockman, B. J. (2018, April) Using NMR-based Activity Assays to Identify Fragment Leads Against Two Trichomonas vaginalis Enzymes, Drug Discovery Chemistry, San Diego, CA.

Stockman, B. J. (2017, April) Biochemical NMR Spectroscopy Course for Upper Level Undergraduates that Combines Both Classroom and Laboratory Learning, American Chemical Society 253rd National Meeting, San Francisco, CA.

Stockman, B. J., Beck, S., Bekker, S. I., Benzie, A. L., Humes, C. S., Muellers, S. N., Rosario Jr., I., Shea, T. A., Violo, V. L., Parkin, D. W. & VanAlstine-Parris, M. A. (2015, May). Nucleoside Ribohydrolases as Targets for Trichomonas vaginalis Therapeutic Agents. American Chemical Society Great Lakes/Central Regional Meeting, Grand Rapids, MI.

Stockman, B. J. (2015, March). NMR-based Activity Assays to Characterize Enzymes in the Biochemistry Laboratory and in Undergraduate Research. 249th American Chemical Society National Meeting, Denver, CO.

Stockman, B. J., Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario Jr., I. & Parkin, D. W. (2013, October). Identification of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Inhibitors Using an 19F NMR-based Activity Assay. American Chemical Society Northeast Regional Meeting, New Haven, CT.

Stockman, B. J. (2013, April). Crowding & Screening: Early Results From Two Undergraduate Research Projects. Hobart and William Smith College, Geneva, NY.

Stockman, B. J. (2010, October). Fragment Screening Using NMR-based Activity Assays. Connecticut College, New London, CT.

Stockman, B. J., Lodovice, I. J., Fisher, D. A., McColl, A. S. & Xie, Z. (2007, January). An NMR-based Functional Assay for NAD Synthetase. Keystone Symposium on Frontiers of NMR in Molecular Biology X, Snowbird, UT.

Stockman, B. J. (2006, September). Adding Value to NMR-Based Fragment Screens By Using Competition Binding or Functional Assays. 232nd American Chemical Society Fall National Meeting, San Francisco, CA.

Stockman, B. J. (2004, November). Competition-Based NMR Binding Assays. Eastern Analytical Symposium, Somerset, NJ.

Stockman, B. J. (2003, March). NMR Screening: Impacting Medicinal Chemistry and Biology. PITTCON 2003, Orlando, FL.

Stockman, B. J. (2000, May). Applications of Flow NMR Spectroscopy to Monitor Binding of Small Molecules to Proteins. NMR in the Drug Discovery Pipeline, London, UK.

Stockman, B. J., Scahill, T. A., Kloosterman, D. A., Jacobsen, E. J., Skaletzky, L. L., Marshall, V. P., Waszak, G. A. & Deibel, M. R. Jr. (1998, August). Solution Structure of Collagenase Complexed with a Lactam Hydroxamate Inhibitor. XVIIIth International Conference on Magnetic Resonance in Biological Systems, Tokyo, Japan.

Stockman, B. J. (1998, May). Matrix Metalloproteinase/Ligand Complexes: Structure, Dynamics, Calorimetry and Inhibitor Design. NATO Advanced Research Workshop: Applications of NMR to the Study of the Structure & Dynamics of Supramolecular Complexes, Sitges, Spain.

Stockman, B. J., Waldon, D. J., Yuan, P., Scahill, T. A., Belonga, K. L., Jacobsen, E. J., Treiberg, J. A., Schostarez, H. J., Mitchell, M. A., Liggett, W. F., Marshall, V. P., Petzold, G. L. & Poorman, R. A. (1996, August). NMR Analysis of Stromelysin/Ligand Complexes as a Tool for Structure Based Drug Design. XVIIth International Conference on Magnetic Resonance in Biological Systems, Keystone, CO.

Stockman, B. J., Scahill, T. A., Euvrard, A., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1993, August). Solution Structure of Human Interleukin-1 Receptor Antagonist Protein Determined by NMR Spectroscopy. 206th American Chemical Society National Meeting, Chicago, IL.

Other Work

Other Work

Listed as inventor or co-inventor on four United States patents:
6,677,160
6,764,858
7,377,894
7,470,543

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